The agglutination assay is used to determine the ability of antibodies to recognize parasite variant antigens on the surface of Plasmodium falciparum -infected erythrocytes. In this technique, infected erythrocytes are selectively labelled with a DNA-binding fluorescent dye and mixed with antibodies of interest to allow antibody-surface antigen binding. Recognition of surface antigens by the antibodies can result in the formation of agglutinates containing multiple parasite-infected erythrocytes. These can be viewed and quantified using a fluorescence microscope.
CITATION STYLE
Tan, J., & Bull, P. C. (2015). Agglutination assays of the Plasmodium falciparum -infected erythrocyte. In Methods in Molecular Biology (Vol. 1325, pp. 115–129). Humana Press Inc. https://doi.org/10.1007/978-1-4939-2815-6_10
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