Antarctic Fish Blood: Respiratory Properties and the Effects Of Thermal Acclimation

Abstract

The effects of thermal acclimation on whole blood oxygen affinity were examined in the antarctic fish Pagothenia borchgrevinki. 4·5 °C-acclimated fish had a P50 value of 26·7 mmHg at pH 8·4, compared to 20·7 mmHg for −1·5 °C-acclimated fish. The apparent heat of oxygenation, ΔH = −26·7 kJ mol−1, is comparable to values for temperate species. Warm-acclimation was followed by an increased ATP:Hb4 molar ratio, resulting in an augmentation of the thermal effect on oxy-haemoglobin affinity. This may be considered adaptive in a constantly well oxygenated environment, where oxygen loading at the gills is secured. Unloading to the tissues is thereby enhanced, supporting an elevated rate of aerobic metabolism at higher temperatures. In vivo blood pH was high, between 8·10 and 8·25 at − 1·5 °C. Astrup titration revealed arterial CO2 tensions of less than 0·8 mmHg, indicating relative hyperventilation and low oxygen extraction efficiency in antarctic fish. Blood oxygen affinities of four antarctic nototheniid species were low (P50 between 11·9 and 20·7 mmHg at pH 8·1 and -1·5 °C) in comparison with the temperate speciesNotothenia angustata (P50 = 10·8 mmHg). The zoarcid Rhigophila dearbomi had a high blood oxygen affinity (P50 = 4·3 mmHg). Blood oxygen-binding properties are discussed in relation to the polar environment, mode of life, and the concept of cold adaptation.