Integration and expression of gfp in the plastid of medicago sativa L

Abstract

Here we describe a protocol of alfalfa (Medicago sativa L.) plastid transformation by which gfp , a gene encoding the green fluorescent protein (GFP), is inserted into plastid genome via particle bombardment and homoplastomic plant is obtained. Plastid engineering is likely to make a significant contribution to the genetic improvement of this crop and the production of vaccines and therapeutic proteins.