Principles of Electroporation for Gene Therapy

Abstract

Gene therapies can compensate for missing or mutated proteins, directly treat cancer, act as cancer or infectious disease vaccines, or modulate protein expression. For any type of gene therapy, the dose of expressed protein should be appropriate to correct the disease. Therapeutic development is often hampered by the concept that an optimal therapy is one with the highest and longest transgene expression coupled with the inability to predict the ultimate expression levels and duration of the therapeutic protein.