Evaluation of a disk method for detection of hippurate hydrolysis by Campylobacter spp.

Abstract

A disk method for detecting hippurate hydrolysis by Campylobacter spp. was evaluated and compared with the conventional tube test used at the Centers for Disease Control (CDC) (G.K. Morris and C.M. Patton, p. 302-308, in E.H. Lennette, A. Balows, W.J. Hausler, Jr., and H.J. Shadomy, ed., Manual of Clinical Microbiology, 4th ed., 1985) and high-performance liquid chromatography (HPLC). A total of 118 Campylobacter strains were tested. Eighty-seven strains (74%) were hippurate positive by the HPLC method, and the remaining 31 (26%) were found to be hippurate negative. By using HPLC as the reference technique, the CDC method showed a sensitivity of 80% and a specificity of 81%; the disk method showed a sensitivity of 93% and a specificity of 94%. The disk method can be performed with a small inoculum of bacteria, did not present problems of interpretation, and showed better results than the CDC method (P = 0.015).