Isolation and Identification of Staphylococcus aureus in Dairy Milk of The Etawah Crossbred Goat with Subclinical Mastitis in Kalipuro Village, Banyuwangi

Abstract

Staphylococcus aureus is one of the bacteria that often causes subclinical mastitis in the Etawah Cross Breed (PE) Goat which causes considerable economic losses due to decreased milk production. This study aimed to isolate and identify Staphylococcus aureus in the milk of PE goats with subclinical mastitis in Kalipuro Village, Banyuwangi. The samples were taken from dairy milk of PE goats on December 2018 in Kalipuro Village, Banyuwangi.Purposive sampling was used for the sampling method which have some criteria such as the cages made of wood and bamboo material, PE goats aged 2-3 years and it were actively milked and evidently positive 3 and 4 subclinical mastitis using Californian Mastitis Test (CMT). As many as 17 samples were taken and then analyzed in laboratory microbiology Faculty of Veterinary Medicine Airlangga University. Phase isolated on MSA media and continued with identification using Gram staining, catalase test, mannitol test, coagulase test and VP test. It showed 16 (94.1%) positive samples of Staphylococcus aureus.