Comparative study between standard pyocyanin and extracted pyocyanin and synergistic with antibiotics against multidrug bacteria isolated from different clinical samples

Abstract

Background: Multidrug Resistance forms in Gram positive and Gram negative bacteria are difficult to treat, and medications to be taken with caution may be unsuccessful. The emergence  of  treat MDR Gram-negative and Gram-positive Infections bacterial is still a major issue. Aims: The goal of this study is reliable  develop new agents or new treatment methods that are capable of overcoming drug resistance in these organisms. Methods:  536(85.3%) samples from out and inpatients who admitted to  Al  Diwaniyah Teaching Hospital in Diwaniyah city  were collected from various site of infection , 92 (46%) Pseudomonas aeruginosa  were investigated by a qualified consulting doctor. After cultivation in special media (MacConkey agar and Nutrient agar) to detect , Pseudomonas aeruginosa  , the samples were identified using the Vitec 2 compact system, Pyocyanin was extracted by standard methods from  Pseudomonas aeruginosa Principal antimicrobial compounds present in pyocyanin were identified by HPLC . Other pathogenic bacteria obtained  for exposure to Pyocyanin like Escherichia coli , K. pneumoniae ,  S. aureus from laboratories  These diagnosis was confirmed by  Vitek 2 system .  Serratia marcescens and Proteus mirabilis  obtained from sputum  patients with co- infection Covid 19.