Flow cytometry is a powerful technique for the detection and quantification of cell surface and intracellular proteins. It enables the ability to measure the expression levels of specific proteins in a cell population of interest without the need to physically separate out the cells from within a heterogeneous population by using the appropriate cell-specific markers. It also requires fewer cells than other traditional techniques such as Western blotting. Here we describe a robust and reproducible method to measure the expression levels of the BCL-2 family members, BCL-2, BCL-XL, and MCL-1 by quantitative flow cytometry (QFCM) using validated antibodies.
CITATION STYLE
Smith, M. L., & Tahir, S. K. (2019). Quantification of BCL-2 Family Members by Flow Cytometry. In Methods in Molecular Biology (Vol. 1877, pp. 163–172). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8861-7_11
Mendeley helps you to discover research relevant for your work.