FTIR and GCMS analysis of Euphorbia hirtaL. and its In-vitroAntibacterial and Antioxidant Activities

Abstract

Introduction: Euphorbia hirtaL. is a common weed plant belongs to family Euphorbiaceae. Thepresent study brings out the importance of weed plants like E. hirtain medicine and health thecare system. Material and Methods:Plant E. hirtahas been collected from thegarden of Department of Botany, University of Delhi and plant leaves were extractedin ethanol by thesoxhlet method. Antibacterial screening has been done by disc-diffusion method. Theantioxidant potential has been determined by TPC, TFC, FRAP, Total antioxidants through phosphomolybdate assay and by DPPH radical scavenging activity. FTIR and GCMS analysis havebeen done for characterization.Result and Discussions:Plant E. hirtashowed significant antibacterial activity against Escherichia coli,Staphylococcus aureus, Bacilus subtilisand Pseudomonas aeruginosawith maximum ZOI diameter 19±0.5mmagainst S.aureusandP.aeruginosaboth. Plant E. hirtaL. showedsignificant antioxidant potential with 196.32 mg/g, 39.7133 mg/g, 595.99 mg/g, 525.84 mg/g values for TPC, TFC, FRAP activity and total antioxidants through phosphomolybdate assay respectively. Plant E. hirtaalso showedsignificant radical scavenging activity against DPPH free radical with amaximum % inhibition 68.505% at 1000μg/ml concentration. FTIR and GCMS of plant E. hirtahave confirmed the presence of various active functional groups and compounds.Conclusion:The current study justifies the importance of E. hirta L. a common weed asasource of natural antioxidants having antibacterial potential and its role in herbal medicines.©2022iGlobal Research and PublishingFoundation. All rights reserved.