As DNA methylation analysis enters the mainstream of biomedical research, there is increasing interest in methodologies that can be used for rapid analysis of a wide variety of biological and clinical samples. The methods that have been commonly used for methylation analysis are usually time-consuming and require multiple steps. The methylation-sensitive high-resolution melting (MS-HRM) assay is an in-tube assay which was developed to assess promoter methylation in sodium bisulphite-modified DNA and is particularly useful for assessing methylation in short fragments of DNA derived from formalin-fixed paraffin-embedded biopsies.
CITATION STYLE
Wong, E. M., & Dobrovic, A. (2011). Assessing Gene-Specific Methylation Using HRM-Based Analysis. In Methods in Molecular Biology (Vol. 687, pp. 207–217). Humana Press Inc. https://doi.org/10.1007/978-1-60761-944-4_14
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