Changes in the S-alk(en)yl cysteine sulfoxides and their biosynthetic intermediates during onion storage

Abstract

Onion (Allium cepa L.) pungency changes during storage. To better understand these flavor changes, seven onion cultivars representing different storage duration, photoperiodic requirement, and flavor intensity were greenhouse grown and the bulbs stored for 3 or 6 months at 5 ± 3°C, 0.8 to 1.1 kPa vapor pressure deficit. Bulbs were evaluated using high-pressure liquid chromatography quantification for changes in S-alk(en)yl cysteine sulfoxide (ACSO) flavor precursors and γ-glutamyl peptide (γ-GP) biosynthetic intermediates before storage and monthly thereafter. Before and during storage, cultivars differed in total ACSO, (+) S-methyl-L-cysteine sulfoxide (MCSO), trans-(+)-S-(1-propenyl)-L-cysteine sulfoxide (PRENCSO), (+) propyI-L-cysteine sulfoxide (PCSO), S-2 carboxypropyl glutathione (2-CARB), and γ-L-glutamyl-S-(1-propenyl)-L-cysteine sulfoxide (γGPECSO) concentration. During storage MCSO generally decreased while PRENCSO increased in concentration for most cultivars. The linear increase in PRENCSO concentration during storage was accompanied by a linear decrease in γGPECSO concentration. While not measured in this study, these trends indicate γglutamyl transpeptidase activity throughout bulb storage. γ-Glutamyl transpeptidase was previously reported to be active only in the later stages of bulb storage or during bulb sprouting. Changes in ACSO and γ-GP compounds during storage did not follow previously reported changes during storage for enzymatically formed pyruvic acid (EPY) for these cultivars. To better understand what causes flavor changes in onions during storage, future investigations should include analysis of the enzymes involved in flavor development and ACSO hydrolysis products.