Abstract
Genetic screens have proven to be a useful tool in the dissection of biological processes in plants. Specifically, suppressor screens have been widely used to study signal transduction pathways. Here we provide a detailed protocol for ethyl methanesulfonate (EMS) mutagenesis used in our suppressor screens in Arabidopsis and discuss the basic principles behind suppressor screen design and downstream analyses.
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APA
Li, X., & Zhang, Y. (2016). Suppressor screens in Arabidopsis. In Methods in Molecular Biology (Vol. 1363, pp. 1–8). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3115-6_1
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