Quantitative nucleic acid amplification by digital PCR for clinical viral diagnostics

10Citations
Citations of this article
31Readers
Mendeley users who have this article in their library.

Abstract

In the past few years, interest in the development of digital PCR (dPCR) as a direct nucleic acid amplification technique for clinical viral diagnostics has grown. The main advantages of dPCR over qPCR include: quantification of nucleic acid concentrations without a calibration curve, comparable sensitivity, superior quantitative precision, greater resistance to perturbations by inhibitors, and increased robustness to the variability of the target sequence. In this review, we address the application of dPCR to viral nucleic acid quantification in clinical applications and for nucleic acid quantification standardization. Further development is required to overcome the current limitations of dPCR in order to realize its widespread use for viral load measurements in clinical diagnostic applications.

Cite

CITATION STYLE

APA

Zhang, K., Lin, G., & Li, J. (2016, September 1). Quantitative nucleic acid amplification by digital PCR for clinical viral diagnostics. Clinical Chemistry and Laboratory Medicine. Walter de Gruyter GmbH. https://doi.org/10.1515/cclm-2015-1101

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free