12th International Congress of Therapeutic Drug Monitoring and Clinical Toxicology

Abstract

Aims: In recent years, several studies investigated the role of single-nucleotide polymorphisms (SNPs) in the follicle-stimulating hormone receptor (FSHR) gene in predicting ovarian response to FSH in IVF/ICSI programs. Most studies were focus on two common SNPs at position 307 or 680 with conflicting results. The objective of this study was to further explore the relationship between FSHR SNPs and ovarian response for patients undergoing IVF/ICSI-ET. Methods: In the retrospective study, 535 patients under 40 years of age undergoing IVF/ICSI-ET procedures were included in this study. Patients with polycystic ovary syndrome, endometriosis, or a previous history of ovarian surgery were excluded. The Sanger DNA sequencing was applied to determine the existence and incidence of FSHR SNPs. Data on ovarian reserve (basal FSH level, ovarian volume, antral follicle count), ovarian responses (peak E2 level, oocytes retrieved, OCCC), FSH dose, number of cleavage, transferable embryos, pregnancy rate were collected. Main outcome measures were response to FSH and FSHR genotype. Results: The frequency genotype of a new non-synonymous SNP (F8L) was 1.1% and 98.9% for FL and LL respectively, whereas at position 307 it was 51.0 %, 37.3% and 11.7% for subjects of TT, TA and AA, and at position 680, it was 74.8 %, 19.6% and 5.6% for NN, NS and SS. The number of cleavage [9.42 € 0.32 for AA, 11.38 € 1.04 for GG at position 307, P = 0.032] and transferable embryos (7.09 € 0.28 for AA, 8.71 € 0.86 for GG at position 307, P = 0.039) were significant lower in TT compared with AA subjects. Ovarian reserve, ovarian response, total FSH dose and pregnancy rate were not significantly different between genotype groups at position 307. None of the clinical parameters were found to be associated with F8L and N680S polymorphisms. Conclusion: In Chinese women, the subjects with AA genotype at position 307 tended to retrieve significantly larger number of cleavage and transferable embryos, but ovarian reserve and response, the FSH dose and pregnancy rate is not associated with F8L, T307A, N680S polymorphisms.