Utilization of Wheat Biorefining Strategy Based on Solid-State Fermentation for Fermentative Production Technique

Abstract

In this study, the development of a new generic feedstock manufacturing technique based on SSF, which was then applied to the succinic acid fermentation process. Separation of the wheat was accomplished using milling and gluten extraction techniques. This waste product from wheat milling, known as bran, was used to manufacture glucoamylase and protease enzymes using Aspergillus oryzae and Aspergillus awamori. A total of 140 g l 1 glucose and more than 3.5 g l 1 free amino nitrogen were produced after hydrolysis of both the gluten-free and gluten-containing solutions. These two sources, when combined, supplied all the nutrients required for succinic acid fermentation mediated by Actinobacillus succinogenes. A fermentation employing just the mixed hydrolysate streams yielded about 22 g l 1 succinic acid. With the addition of MgCO3 to the wheat-derived medium, succinic acid production rose to 64 g l 1. Using the SSF-based technology, it was possible to create a generic wheat feedstock that could be utilised to make succinic acid and then demonstrate the effectiveness of the proposed methodology.