GCK gene-body hypomethylation is associated with the risk of coronary heart disease

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Abstract

Objectives. Glucokinase encoded by GCK is a key enzyme that facilitates phosphorylation of glucose to glucose-6-phosphate. Variants of GCK gene were shown to be associated with type 2 diabetes (T2D) and coronary heart disease (CHD). The goal of this study was to investigate the contribution of GCK gene-body methylation to the risk of CHD. Design and Methods. 36 patients (18 males and 18 females) and 36 age- and sex-matched controls were collected for the current methylation research. DNA methylation level of the CpG island (CGI) region on the GCK gene-body was measured through the sodium bisulfite DNA conversion and pyrosequencing technology. Results. Our results indicated that CHD cases have a much lower methylation level (49.77 ± 6.43%) compared with controls (54.47 ± 7.65%, P=0.018). In addition, GCK gene-body methylation was found to be positively associated with aging in controls (r=0.443, P=0.010). Conclusions. Our study indicated that the hypomethylation of GCK gene-body was significantly associated with the risk of CHD. Aging correlates with an elevation of GCK methylation in healthy controls. © 2014 Limin Xu et al.

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Xu, L., Zheng, D., Wang, L., Jiang, D., Liu, H., Xu, L., … Duan, S. (2014). GCK gene-body hypomethylation is associated with the risk of coronary heart disease. BioMed Research International, 2014. https://doi.org/10.1155/2014/151723

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