Amyloid fibrils are attractive targets for applications in biotechnology. These thin, nanoscale protein fibers are highly ordered structures that self-assemble from their component proteins or peptides. This chapter describes the use of several biophysical techniques to monitor the formation of amyloid fibrils including a common dye-binding assay, turbidity assay, and small-angle X-ray scattering. These techniques provide information about the assembly mechanism, the rate and reproducibility of assembly, as well as the size of species along the assembly pathway. © 2013 Springer Science+Business Media, New York.
CITATION STYLE
Sawyer, E. B., & Gras, S. L. (2013). Self-assembling nanomaterials: Monitoring the formation of amyloid fibrils, with a focus on small-angle x-ray scattering. Methods in Molecular Biology, 996, 77–101. https://doi.org/10.1007/978-1-62703-354-1_5
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