Expression of keratins 8, 18, and 19 in epithelia of atrophic oral lichen planus

14Citations
Citations of this article
12Readers
Mendeley users who have this article in their library.

Abstract

Keratins form intermediate filaments of the cytoskeleton in keratinocytes and have roles in cell structure, signaling, intracellular transport, and cell death. Oral lichen planus (OLP) is an oral inflammatory disease with derangements in basal keratinocytes and disruption of the basal membrane. Here, we focused on epithelial expression of keratins 8, 18, and 19 because these proteins are known to modulate cell death. Biopsies were taken from buccal oral mucosa of persons with normal oral mucosa (n = 10) or atrophic OLP (n = 10). Cultured normal oral keratinocytes (n = 4) showed expression of mRNA and protein for keratins 8, 18, and 19. Immunohistochemistry showed consistent staining for keratins 8 and 18 in basal keratinocytes of normal oral mucosa. In OLP, staining for keratin (K)8 was mostly negative and staining for K18 was weak. Keratin 19 was expressed irregularly in most biopsies of normal oral mucosa and not at all in OLP. Several mononuclear leukocytes in the cellular infiltrate showed membrane staining for K8 and K18. Positive staining for K16 confirmed partial collapse of the basal cell layer in OLP. The basal cell niche in OLP therefore appeared to be partly populated with keratinocytes demonstrating a higher degree of differentiation (K8−K18−K19−K16+); consequently, such areas may be more susceptible to the action of cell death factors released from the cell infiltrate as a result of lacking the protective, normal keratin present in the basal epithelial cell layer of normal oral mucosa.

References Powered by Scopus

The human keratins: Biology and pathology

1154Citations
N/AReaders
Get full text

Intermediate Filaments: Molecular structure, assembly mechanism, and integration into functionally distinct intracellular scaffolds

587Citations
N/AReaders
Get full text

Immunocytochemical detection and mapping of a cytokeratin 18 neo- epitope exposed during early apoptosis

587Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Roles of the cytoskeleton in human diseases

13Citations
N/AReaders
Get full text

Composition of hemidesmosomes in basal keratinocytes of normal buccal mucosa and oral lichen planus

6Citations
N/AReaders
Get full text

RvD1<inf>n-3 DPA</inf> Downregulates the Transcription of Pro-Inflammatory Genes in Oral Epithelial Cells and Reverses Nuclear Translocation of Transcription Factor p65 after TNF-α Stimulation

5Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Schreurs, O., Karatsaidis, A., Balta, M. G., Grung, B., Hals, E. K. B., & Schenck, K. (2020). Expression of keratins 8, 18, and 19 in epithelia of atrophic oral lichen planus. European Journal of Oral Sciences, 128(1), 7–17. https://doi.org/10.1111/eos.12666

Readers' Seniority

Tooltip

Professor / Associate Prof. 2

50%

PhD / Post grad / Masters / Doc 2

50%

Readers' Discipline

Tooltip

Medicine and Dentistry 2

33%

Immunology and Microbiology 2

33%

Mathematics 1

17%

Chemistry 1

17%

Article Metrics

Tooltip
Social Media
Shares, Likes & Comments: 20

Save time finding and organizing research with Mendeley

Sign up for free