Ribosome display is a very effective and powerful technology for screening functional peptides or polypeptides in vitro. In ribosome display, each peptide or polypeptide (phenotype) links with its corresponding mRNA (genotype) through a ribosome. This link can be achieved by the absence of a stop codon in the mRNA, therefore stalling the ribosome at the end of translation with the nascent random sequence peptide extended by a spacer outside of the ribosome tunnel. In this chapter, we describe a method for the use of a further stabilized peptide-ribosome-mRNA complex for ribosome display. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Hara, S., Liu, M., Wang, W., Xu, M., Li, Z., & Ito, Y. (2012). Stabilized ribosome display for in vitro selection. Methods in Molecular Biology, 805, 59–73. https://doi.org/10.1007/978-1-61779-379-0_4
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