A quantitative reverse transcriptase-polymerase chain reaction assay to identify metastatic carcinoma tissue of origin

98Citations
Citations of this article
36Readers
Mendeley users who have this article in their library.

Your institution provides access to this article.

Abstract

Identifying the primary site in patients with metastatic carcinoma of unknown primary origin can enable more specific therapeutic regimens and may prolong survival. Twenty-three putative tissue-specific markers for lung, colon, pancreatic, breast, prostate, and ovarian carcinomas were nominated by querying a gene expression profile database and by performing a literature search. Ten of these marker candidates were then selected based on validation by reverse transcriptase-polymerase chain reaction (RT-PCR) on 205 formalin-fixed, paraffin-embedded metastatic carcinoma specimens originating from these six and from other cancer types. Next, we optimized the RNA isolation and quantitative RT-PCR methods for these 10 markers and applied the quantitative RT-PCR assay to a set of 260 metastatic tumors. We then built a gene-based algorithm that predicted the tissue of origin of metastatic carcinomas with an overall leave-one-out cross-validation accuracy of 78%. Lastly, our assay demonstrated an accuracy of 76% when tested on an independent set of 48 metastatic samples, 37 of which were either a known primary or initially presented as carcinoma of unknown primary but were subsequently resolved. Copyright © American Society for Investigative Pathology and the Association for Molecular Pathology.

Cite

CITATION STYLE

APA

Talantov, D., Baden, J., Jatkoe, T., Hahn, K., Yu, J., Rajpurohit, Y., … Mazumder, A. (2006). A quantitative reverse transcriptase-polymerase chain reaction assay to identify metastatic carcinoma tissue of origin. Journal of Molecular Diagnostics, 8(3), 320–329. https://doi.org/10.2353/jmoldx.2006.050136

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free