Bioremediation Studies on Melanoidin Containing Distillery Spent Wash by Using Leuconostoc mesenteroides

Abstract

Melanoidins are the natural condensation products present in distillery spent wash. It is the result of Millard reaction which occurs between glucose and glycine. From environmental aspects, melanoidins are very important because of the complex structure and caliginous colour and objectionable odour, which can cause damage to the surroundings. All these characteristics of melanoidin modify the photosynthetic activity of plants, reduce the dissolved oxygen of aquatic ecosystem, soil fertility is affected to a large extent, and it also slows down the sprouting of seeds. Bioremediation is an environmentally friendly technology for the treatment of chemical and hazardous waste. Bacteria from the nearby contaminated soil have a high potential to convert complex bioorganic substances to simpler absorbable form. With reference to this utilization of bacteria, Leuconostoc mesenteroides is an economical technology for the treatment of contaminated water having melanoidin. After treatment, the distillery spent wash was tested by using the analytical techniques as spectrophotometry, modified spectroscopy as Fourier-transform infrared (FTIR), chromatography with high performance i.e. (HPLC). In the present research paper, the results of above-mentioned techniques helped to make conclusions, as distillery spent wash polluted site contains a novel microorganism, which would be effective for the decolourisation as well as degradation of distillery spent wash. Application of Leuconostoc mesenteroids showed 40% distillery spent wash which contains melanoidin. Thus, the use of this novel microorganism in the field of environmental biotechnology will be an effective way to solve the biggest problem of water and soil pollution.