B cells, after activation, can undergo class-switch recombination and somatic hypermutation of their immunoglobulin genes, and can differentiate into memory cells and plasma cells. Expressing genes in altered versions in primary B cells and B cell lines is an important approach to understanding how B cell receptor signaling leads to B cell activation and differentiation. Recombinant retrovirus-based transduction is the most efficient method to deliver exogenous genes for expression in B cells. In this chapter, we describe streamlined protocols for using recombinant retroviral vectors to transduce both murine primary B cells and B cell lines.
CITATION STYLE
Yang, Z., & Allen, C. D. C. (2018). Expression of exogenous genes in murine primary B cells and B cell lines using retroviral vectors. In Methods in Molecular Biology (Vol. 1707, pp. 39–49). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7474-0_3
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