Receptor-based nucleic acid sensing constitutes one of the most fundamental mechanisms of our innate immune system to sense viral infection. RIG-I is a cytosolic RNA helicase that senses the presence of 5' triphosphate RNA species, a common feature of many negative strand RNA viruses. We here describe a protocol to enzymatically synthesize and to purify a defined RIG-I ligand that can be used to study RIG-I activation in vitro and in vivo.
CITATION STYLE
Goldeck, M., Schlee, M., Hartmann, G., & Hornung, V. (2014). Enzymatic synthesis and purification of a defined RIG-I Ligand. Methods in Molecular Biology, 1169, 15–25. https://doi.org/10.1007/978-1-4939-0882-0_2
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