Xanthomonas campestris as a host for the production of recombinant Pseudomonas aeruginosa lipase

Abstract

Recombinant plasmid pBP13, which expresses the alkaline lipase from Pseudomonas aeruginosa IGB83 under the tac promoter was transferred to Xanthomonas campestris pv campestris IBT148. Different fermentation conditions were tested for lipase productivity by strain IBT148 carrying plasmid pBP13, and a fermentation process was established in an instrumented bioreactor, where lipase production was increased more than 12-fold with respect to the initial culture conditions in shake flasks, Xanthan gum stabilized the activity of the alkaline lipase.