This chapter describes protocols for mass spectrometry (MS) applied to the characterization of ganglioside structures and the determination of ganglioside contents. Matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) are often used to ionize biological materials and this chapter covers three protocols for atmospheric pressure MALDI MS (AP-MALDI MS), liquid chromatography-ESI MS (LC-ESI MS), and LC-ESI MS with multiple reaction monitoring (MRM). Purified gangliosides were used in AP-MALDI MS analyses while crude preparations of gangliosides were subjected to LC-ESI MS and LC-ESI MS with MRM. The LC protocol includes conditions for both reversed-phase and normal-phase column chromatography.
CITATION STYLE
Suzuki, A., Suzuki, M., Ito, E., Nitta, T., & Inokuchi, J. ichi. (2018). Mass spectrometry of gangliosides. In Methods in Molecular Biology (Vol. 1804, pp. 207–221). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8552-4_9
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