Ferroptosis is a new form of cell death with hallmark of lipid peroxidation and iron accumulation. It has been shown that lipid peroxidation can result in electrophilic metabolites which in turn induce protein carbonylations. Identification of specific carbonylated proteins and sites in ferroptotic cells will be of great significance for understanding the mechanism and discovering potential biomarkers for this new cell death. The protocol described herein is an optimized pipeline which combines the labeling of carbonylated proteins by a commercially available aniline-based probe with the tandem orthogonal proteolysis activity-based protein profiling (TOP-ABPP) strategy to portrait the landscape of carbonylations in ferroptotic cells.
CITATION STYLE
Chen, Y., & Wang, C. (2022). Profiling of Protein Carbonylations in Ferroptosis by Chemical Proteomics. In Methods in Molecular Biology (Vol. 2543, pp. 141–153). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2553-8_12
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