Abstract
One kojibiose phoshorylase (KP) homolog gene was cloned from Caldicellulosiruptor saccharolyticus ATCC43494. Recombinant KP from C. saccharolyticus (Cs-KP) expressed in Escherichia coli showed highest activity at pH 6.0 at 85 °C, and was stable from pH 3.5 to 10.0 and up to 85 °C for phosphorolysis. Cs-KP showed higher productivity of kojioligosaccharides of DP ≥ 4 than KP from Thermoanaerobacter brockii ATCC35047.
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Yamamoto, T., Nishio-Kosaka, M., Izawa, S., Aga, H., Nishimoto, T., Chaen, H., & Fukuda, S. (2011). Enzymatic properties of recombinant kojibiose phosphorylase from Caldicellulosiruptor saccharolyticus ATCC43494. Bioscience, Biotechnology and Biochemistry, 75(6), 1208–1210. https://doi.org/10.1271/bbb.110116
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