In vitro culture and differentiation of osteoblasts on coral scaffold from human bone marrow mesenchymal stem cells

Abstract

Biomaterial such as corals is an excellent osteoconductive material to be used to bone human derived stem cells for clinical regenerative intent. Bone regeneration is often needed for multiple clinical purposes for instance in aesthetic reconstruction and regenerative procedures. In this paper we describe an approach that aim to provide fundamental information allowing a scientific biomechanical basis for use of natural coral scaffold to initiate mesenchymal stem cells into osteogenic differentiation process for transplant purposes. Isolated MSCs from human bone marrow were induced into osteoblasts by using an osteogenic medium enriched with two specific growth factors FGF9 and vitamin D2. Eventually, part of cultured MSCs were directly transferred and seeded onto coral scaffolds and induced to differentiate into osteoblasts and part were cultured in apposite flask for mesenchymal stem cell medium. Finally, our data indicated that hBM is a very reliable source of MSCs and that these cells may be easily differentiated into osteoblast and seeded into coral as optimal device for clinical application. © Springer-Verlag 2010.