Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila has been a powerful system to explore the functions of HSPGs in vivo. In this chapter, we will introduce two genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting) and MARCM (mosaic analysis with a repressible cell marker).
CITATION STYLE
Takemura, M., & Nakato, H. (2015). Genetic approaches in the study of heparan sulfate functions in drosophila. Methods in Molecular Biology, 1229, 497–505. https://doi.org/10.1007/978-1-4939-1714-3_38
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