Nerve growth factor (NGF), a neurotrophin critical to neuronal viability, has become a popular research focus for the treatment of neurodegenerative diseases. Enzyme-linked immunosorbent assays (ELISA) allow the quantification of cellular proteins, such as NGF, secreted into conditioned culture media. However, off-the-shelf reagents and kit components may require optimization depending on the specific specimens and/or antibodies that will be utilized with the ELISA assay. Herein we describe a protocol for developing a sandwich ELISA, incorporating NGF-specific primary and secondary antibodies capable of detecting NGF at concentrations as low as 10 pg/mL.
CITATION STYLE
Davis, J. B. (2017). ELISA for monitoring nerve growth factor. In Methods in Molecular Biology (Vol. 1606, pp. 141–147). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6990-6_10
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