Evaluation in vitro of cytotoxicity of dentinal desensitizing on human gingival fibroblasts.

Abstract

Introduction: The purpose of this study is to compare the cytotoxic effect of three materials which have been used for the treatment of dental hypersensitivity. Material and method: In-vitro study. Dentinal desensitizing Clinpro (3M ESPE), Seal&Protect (Dentsply) and UltraEZ (Ultradent) were used at concentrations of 0.1; 0.05; 0.01 and 0.001 g/ml against human gingival fibroblasts. Furthermore, Clinpro and Seal&Protect were applied to this cell culture as polymerized discs. Toxicity was assessed at 24 and 48 hours by cell viability assay (MTT). Statistical analysis for cell viability was performed using ANOVA two-ways and Tukey's post hoc test. Statistical significance was set at 5%. Results: Seal&Protect and Clinpro happened to be highly toxic at 24 and 48 hours, reaching 70% toxicity at concentrations over 0,01 g/ml. Seal&Protect and Clinpro polymerized discs were toxic after 24 and 48 hours. UltraEZ was increase of the cell viability between a 46% and 67% at 24 hours and between an 8% and 45% at 48 hours. Statistical analysis showed differences between these three desensitizing comparing concentration and control group (p<0,05). Discussion: UltraEZ does not have cytotoxic effect and may be considered a compatible and safe material to be utilized, whereas polymerized and non-polymerized Clinpro and Seal&Protect should be used with caution.