MicroRNAs (miRNAs) are small non-coding RNAs that mediate post-transcriptional gene silencing. Over 700 human miRNAs have currently been identified, many of which are mutated or de-regulated in diseases. Here we report the identification of novel miRNAs through deep sequencing the small RNAome (<30 nt) of over 100 tissues or cell lines derived from human female reproductive organs in both normal and disease states. These specimens include ovarian epithelium and ovarian cancer, endometrium and endometriomas, and uterine myometrium and uterine smooth muscle tumors. Sequence reads not aligning with known miRNAs were each mapped to the genome to extract flanking sequences. These extended sequence regions were folded in silico to identify RNA hairpins. Sequences demonstrating the ability to form a stem loop structure with low minimum free energy (
CITATION STYLE
Creighton, C. J., Benham, A. L., Zhu, H., Khan, M. F., Reid, J. G., Nagaraja, A. K., … Gunaratne, P. H. (2010). Discovery of novel MicroRNAs in female reproductive tract using next generation sequencing. PLoS ONE, 5(3). https://doi.org/10.1371/journal.pone.0009637
Mendeley helps you to discover research relevant for your work.