Conventional oxygen (micro-) sensors assess oxygen concentration within a particular region or across a transect of tissue, but provide no information regarding its bidimensional distribution. Here, a novel imaging technology is presented, in which an optical sensor foil (i.e., the planar optode) is attached to the surface of the sample. The sensor converts a fluorescent signal into an oxygen value. Since each single image captures an entire area of the sample surface, the system is able to deduce the distribution of oxygen at a resolution level of few micrometers. It can be deployed to dynamically monitor oxygen consumption, thereby providing a detailed respiration map at close to cellular resolution. Here, we demonstrate the application of the imaging tool to developing plant seeds; the protocol is explained step by step and some potential pitfalls are discussed.
CITATION STYLE
Rolletschek, H., & Liebsch, G. (2017). A method for imaging oxygen distribution and respiration at a microscopic level of resolution. In Methods in Molecular Biology (Vol. 1670, pp. 31–38). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7292-0_3
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