Production of antibody fragments using the baculovirus-insect cell system

Abstract

The baculovirus-insect cell system is effective in the production of large quantities of biologically active recombinant proteins. The secretory production of an antibody Fab fragment in lepidopteran insect cells infected with a recombinant baculovirus that contains both the heavy-chain (Hc; Fd fragment) and light-chain (Lc) genes of the Fab fragment is described in this chapter. The recombinant baculovirus can be generated rapidly and efficiently through site-specific transposon-mediated insertion of foreign genes into a baculovirus genome called a bacmid in Escherichia coli. Factors influencing the Fab fragment production are discussed. © 2012 Springer Science+Business Media, LLC.