Optimizing the conditions for the overexpression of membrane proteins in E. coli and their subsequent purification is usually a laborious and time-consuming process. Combining the Lemo21(DE3) strain, which conveniently allows to identify the optimal expression intensity of a membrane protein using only one strain, and membrane proteins C-terminally fused to Green Fluorescent Protein (GFP) greatly facilitates the production of high-quality membrane protein material for functional and structural studies. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Hjelm, A., Schlegel, S., Baumgarten, T., Klepsch, M., Wickström, D., Drew, D., & De Gier, J. W. (2013). Optimizing E. coli-based membrane protein production using Lemo21(DE3) and GFP-fusions. Methods in Molecular Biology, 1033, 381–400. https://doi.org/10.1007/978-1-62703-487-6_24
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