Quantitative Fluorescence Calibration: a Tool for Assessing the Quality of Data Obtained by Fluorescence Measurements

Abstract

Over the last three decades, fluorescence has become the most widely used detection method in biomedical science. Fluorescence measurements are utilized in basic and translational research, method development, medical laboratory tests, clinical trials and epidemiologic studies. This renders the availability of objective methods to assess the quality of data obtained by these measurements very critical for research scientists, clinical laboratory personnel, and regulatory reviewers as is addressed in this chapter.