Molecular cloning and sequence of the gene for outer membrane protein P5 of Haemophilus influenzae

Abstract

The gene for outer membrane protein P5 of Haemophilus influenzae was identified by immunological screening of a genomic λEMBL3 library of the serotype b strain 1613. The gene was subcloned, and plasmid clones expressing P5 were identified by immunologic screening. The gene for outer membrane protein P5 was sequenced. The mature protein has a molecular weight of 35,628. The protein is 50% identical and 65% similar to the OmpA protein of Escherichia coli.