The molecular characterization of the protein and RNA factors that are required for Sec incorporation in mammals has been largely carried out using in vitro translation systems specifically modified for this purpose. This chapter outlines the various systems and modifications that have been used to decipher the mechanism of Sec incorporation.
CITATION STYLE
Pinkerton, M. H., & Copeland, P. R. (2018). In vitro translation assays for selenocysteine insertion. In Methods in Molecular Biology (Vol. 1661, pp. 93–101). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7258-6_7
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