During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10,000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near-patient settings and can be performed by medical personnel with little prior experience in qPCR.
CITATION STYLE
Pavletić, M., Mazor, M., Lerga, M., Mileta, T., Železnjak, J., Ružić, T., … Lisnić, V. J. (2021). Fast, reliable, and simple point-of-care-like adaptation of RT-qPCR for the detection of SARS-CoV-2 for use in hospital emergency departments. Viruses, 13(12). https://doi.org/10.3390/v13122413
Mendeley helps you to discover research relevant for your work.