Fast, reliable, and simple point-of-care-like adaptation of RT-qPCR for the detection of SARS-CoV-2 for use in hospital emergency departments

1Citations
Citations of this article
22Readers
Mendeley users who have this article in their library.

Abstract

During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10,000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near-patient settings and can be performed by medical personnel with little prior experience in qPCR.

Cite

CITATION STYLE

APA

Pavletić, M., Mazor, M., Lerga, M., Mileta, T., Železnjak, J., Ružić, T., … Lisnić, V. J. (2021). Fast, reliable, and simple point-of-care-like adaptation of RT-qPCR for the detection of SARS-CoV-2 for use in hospital emergency departments. Viruses, 13(12). https://doi.org/10.3390/v13122413

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free