Confirming the binding of a transcription factor with a particular DNA sequence may be important in characterizing interactions with a synthetic promoter. Electrophoretic mobility shift assay is a powerful approach to demonstrate the specific DNA sequence that is bound by a transcription factor and also to confirm the specific transcription factor involved in the interaction. In this chapter we describe a method we have successfully used to demonstrate interactions of endogenous transcription factors with sequences derived from endogenous and synthetic promoters.
CITATION STYLE
Yousaf, N., & Gould, D. (2017). Demonstrating interactions of transcription factors with DNA by electrophoretic mobility shift assay. In Methods in Molecular Biology (Vol. 1651, pp. 11–21). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7223-4_2
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