Anti-MDR bacterial activity of bacteria associated with sea sponge of Amphimedon sp. from Karimunjawa Island, Central Java, Indonesia

Abstract

Aims: The aim of this study was to evaluate the antibacterial activity of bacteria associated with sea sponge Amphimedon sp. against multi-drug resistant (MDR) bacteria that cause wound infections. Methodology and results: The antibacterial activity was evaluated by the overlay method. Identification of active bacterial symbionts was carried out using the 16S rRNA gene sequence-based method of bacterial identification. The results suggest that one of nine isolates had antibacterial activity against MDR bacteria. Isolate Z9VIII was identified as Bacillus subtilis and demonstrated robust antibacterial activity against bacteria: MRSA (11 mm), MDR-AB (17 mm) and CRPA (12 mm). Conclusion, significance and impact of study: This study concludes that the one of the bacterial species associated with the sea sponge of Amphimedon sp. was B. subtilis, which has the potential as antibacterial agent against MDR bacteria.