Production Optimization and Characterization of Bioactive Compound against Salmonella bacillus subtilis KBB Isolated from Nepal

Abstract

The present study aimed to isolate bioactive Bacillus spp. against multidrug resistant Salmonella and many other gram-negative bacterial pathogens. Altogether five bioactive Bacillus were isolated from soil samples of Nepal and identified as Bacillus licheniformis, Bacillus subtilis, Bacillus pumilus, Bacillus sp. and Bacillus cereus respectively by conventional techniques and DNA sequencing of the 16S rRNA gene. In all five isolates, the isolate Bacillus subtillis KBB was most potent antagonist of multidrug resistant Salmonella as tested by agar disc diffusion method. The bioactive compound production was optimized and product was purified by TLC, and bioactive molecule was characterized UV, IR NMR and GCMS and identified as peptide compound. Key words: Bacillus; Bioactivity; Production optimization; Peptide compounds; One step solvent extraction; Antibacterial spectrum. DOI: 10.3126/sw.v8i8.3841 Scientific World Vol.8(8) 2010 pp.19-29