Ultrasmall compact CMOS imaging system for bioluminescence reporter-based live gene expression analysis

  • Olorocisimo J
  • Briones J
  • Sasagawa K
  • et al.
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Abstract

Significance: Gene expression analysis is an important fundamental area of biomedical research. However, live gene expression imaging has proven challenging due to constraints in conventional optical devices and fluorescent reporters. Aim: Our aim is to develop smaller, more cost-effective, and versatile imaging capabilities compared with conventional devices. Bioluminescence reporter-based gene expression analysis was targeted due to its advantages over fluorescence-based imaging. Approach: We created a small compact imaging system using micro-CMOS image sensors (μCIS). The μCIS model had an improved pixel design and a patterned absorption filter array to detect the low light intensity of bioluminescence. Results: The device demonstrated lower dark current, lower temporal noise, and higher sensitivity compared with previous designs. The filter array enabled us to subtract dark current drift and attain a clearer light signal. These improvements allowed us to measure bioluminescence reporter-based gene expression in living mammalian cells. Conclusion: Using our μCIS system for bioluminescence imaging in the future, the device can be implanted in vivo for simultaneous gene expression imaging, behavioral analysis, and optogenetic modulation.

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APA

Olorocisimo, J. P., Briones, J., Sasagawa, K., Haruta, M., Takehara, H., Tashiro, H., … Ohta, J. (2021). Ultrasmall compact CMOS imaging system for bioluminescence reporter-based live gene expression analysis. Journal of Biomedical Optics, 26(11). https://doi.org/10.1117/1.jbo.26.11.116002

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