cDNA encoding human carbonic anhydrase II has been isolated and its nucleotide sequence determined. Expression of the isolated carbonic anhydrase gene in Escherichia coli from a plasmid containing the tac promoter yielded an active enzyme at a level of about 1% of total protein.
CITATION STYLE
Forsman, C., Behravan, G., Osterman, A., & Jonsson, B. H. (1988). Production of active human carbonic anhydrase II in E. coli. Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry, 42(5), 314–318. https://doi.org/10.3891/acta.chem.scand.42b-0314
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