Methanol Extracts Formulation of Tambora Leaves (Ageratum conyzoides L.), Sembalit Angin Leaves (Mussaendafrondosa L.) and Turmina Rhizome (Curcuma longa) as Candida albicans Antifungal

Abstract

This study aimed to analyze the combination formula of Tambora leaves, Sembalit Angin leaves, and Turmeric Rhizomes using a 2: 1: 3 ratio, and determine its effect on the growth of Candida albicans. The growth indicator was measured based on the growth area of inhibition zone which marked by a clear zone between the outer sides of the paper disc. The growth activity was measured at the incubation period of 1x24 hours, 2x24 hours, and 3x24 hours. The observation data then were analyzed by oneway ANOVA statistical test continued with the Duncan test 1%. The results showed that the combination formula of bioherbal methanol extract of Tambora leaves, Sembalit Angin leaves, and turmeric rhizomes had a significant effect on the growth of Candida albicansat 1% significance, proven by the sig value. 0.00 <0.01. Therefore, the combination formula of bioherbal methanol extract of Tambora leaves, Sembalit Angin leaves, and Turmeric rhizomes can be recommended as a combination formula extract that can inhibit the growth of Candida albicans.