Regeneration of Coronilla varia L. (crownvetch) plants from callus culture

Abstract

Coronilla varia L. (crownvetch) plants were regenerated from callus cultures through somatic embryogenesis. Callus cultures were initiated using hypocotyls excised from sterile seedlings. Cultures were then transferred from a modified Gamborg's B5 medium containing 2,4-D to a medium containing no plant growth regulators (basal B5). Formation of embryos was evident in 12 of 32 callus lines after transfer of callus to BOi2Y (modified Blaydeś medium supplemented with 100 mg inositol and 2 g yeast extract/L). Basal B5 supplemented with 10 mM asparagine or 20 mM NH4Cl could be substituted for BOi2Y. Embryos subsequently transferred to basal B5 developed roots and shoots. Plants thus formed were first transferred to vermiculite and then to soil. © 1984 Martinus Nijhoff/Dr W. Junk Publishers.