Analysis of intact proteins by tandem mass spectrometry has mostly been confined to high-end mass spectrometry platforms. This protocol describes the application of routine HPLC to separate proteins, MALDI-ToF mass spectrometry to interrogate intact protein species and electron transfer dissociation/proton transfer reaction within a quadrupole ion trap to perform tandem mass spectrometry.
CITATION STYLE
Hart, S. R. (2010). A Protocol for top-down proteomics using HPLC and ETD/PTR-MS. Methods in Molecular Biology (Clifton, N.J.), 658, 339–353. https://doi.org/10.1007/978-1-60761-780-8_21
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