Chromatin organization in pluripotent cells: Emerging approaches to study and disrupt function

3Citations
Citations of this article
75Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Translating the vast amounts of genomic and epigenomic information accumulated on the linear genome into threedimensional models of nuclear organization is a current major challenge. In response to this challenge, recent technological innovations based on chromosome conformation capture methods in combination with increasingly powerful functional approaches have revealed exciting insights into key aspects of genome regulation. These findings have led to an emerging model where the genome is folded and compartmentalized into highly conserved topological domains that are further divided into functional subdomains containing physical loops that bring cis-regulatory elements to close proximity. Targeted functional experiments, largely based on designable DNA-binding proteins, have begun to define the major architectural proteins required to establish and maintain appropriate genome regulation. Here, we focus on the accessible and wellcharacterized system of pluripotent cells to review the functional role of chromatin organization in regulating pluripotency, differentiation and reprogramming.

Cite

CITATION STYLE

APA

Novo, C. L., & Rugg-Gunn, P. J. (2016). Chromatin organization in pluripotent cells: Emerging approaches to study and disrupt function. Briefings in Functional Genomics, 15(4), 305–314. https://doi.org/10.1093/bfgp/elv029

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free