Growing adipose-derived stem cells (ADSC) in serum-free conditions is important as it represents a way of expanding multipotent cells in a clinical grade medium. Most cultured ADSC are expanded and tested in serum-containing media, which can pose significant health risks if these cells were used in clinical applications. Moreover, cells grown in serum-free conditions behave significantly different than those cultured in serum-containing media. Here, we present a technique to culture adipose-derived stem cells in serum-free conditions. The methods described in this chapter were optimized for ovine ADSC. The appropriate optimization should be done for other cell lines.
CITATION STYLE
Zanicotti, D. G., & Coates, D. E. (2017). Growing adipose-derived stem cells under serum-free conditions. In Methods in Molecular Biology (Vol. 1537, pp. 439–446). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6685-1_26
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