DNA barcoding of gastropods Terebralia semistriata (Mörch, 1852 (Potamididae: Gastropoda)

Abstract

Potamididae: Gastropods are grouped as one of family belongs to class gastropods which is found very abundant in tropical intertidal areas, especially in mangrove ecosystems. Apart from being self defense (protection), the shells of Potamididae gastropods are also useful as a key species for identification. This study aims to identify Potamididae gastropods through a barcoding DNA approach using JgLCO1490 and JgHCO2198 primers. The results of identification based on morphological characters, revealed that the Potamididae gastropods analyzed were Terebralia sp. Furthermore, identification using the barcoding DNA approach, show that the Potamididae gastropods were a type of Terebralia semistriata with a 98.93% similarity rate. The length of DNA sequence amplified with the COI gene was 655 bp. Phylogenetic analysis and genetic distance, the DNA sequences of Terebralia semistriata from Merauke form the same clade as Terebralia semistriata from Queensland, Australia with genetic distance of 0.1%. Identification species using the DNA barcoding approach makes identification of organisms easier, faster and more accurate.